Optical detection of molecular beacons in microfluidic devices

TitleOptical detection of molecular beacons in microfluidic devices
Publication TypeConference Paper
Year of Publication2000
AuthorsM Balberg, R Hristova, DJ Brady, DJ Beebe, and L Raskin
Conference Name1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
Date Published01/2000

Hybridization and detection of E. coli ribosomal RNA with molecular beacons labeled with a fluorescent dye Is achieved in a microfluidic device. The device consists of a four ports mixing chamber, where the probes and the target molecules mix by diffusion. The weak fluorescent signal is detected by two large core optical fibers that are placed on both sides of the channel and collect the light emitted in both directions. The fibers are coupled to a spectrophotometer. The detection limit for the system is 0.2 ftnol.