Identification of fluorescent beads using a coded aperture snapshot spectral imager.

TitleIdentification of fluorescent beads using a coded aperture snapshot spectral imager.
Publication TypeJournal Article
Year of Publication2010
AuthorsCF Cull, K Choi, DJ Brady, and T Oliver
JournalApplied Optics
Volume49
Start PageB59
Issue10
PaginationB59 - B70
Date Published04/2010
Abstract

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead identification from CASSI measurements by combining a priori knowledge of the spectra associated with each bead type. Our proposed method creates a 2D bead identity image. Simulated fluorescence CASSI measurements are used to evaluate the behavior of the algorithm. We also record real CASSI measurements of a ten bead type fluorescence scene and create a 2D bead identity map. A baseline image from filtered-array imaging system verifies CASSI's 2D bead identity map.

DOI10.1364/ao.49.000b59
Short TitleApplied Optics